Protein sequencing by ISD and PSD MALDI-TOF MS

Hepatocellular carcinoma-associated protein markers investigated by MALDI-TOF MS.

Hepatocellular carcinoma (HCC) is one of the most common types of cancer worldwide. The initial hepatocellular alterations that precede the appearence of HCC include chronic viral hepatitis/cirrhosis, foci of phenotypically altered hepatocytes and, subsequently, dysplastic hepatocytes that form foci and nodules. These changes cause a discrepancy in the microenvironment of liver cells, which may...

Dossier MALDI - TOF ■ Oligonucleotide analysis by MALDI - MS

Matrix-assisted laser desorption (MALDI) time-offlight (TOF) mass spectrometry (MS) has been successfully used for oligonucleotide sequence confirmation by mass analysis of fragments generated by concentration-dependent exonuclease digestion. This approach is particularly well-suited to the analysis of synthetic DNA due to the relatively short length of the DNA strands. Additionally, an importa...

Extraction and sequencing of human and Neanderthal mature enamel proteins using MALDI-TOF/TOF MS

We report here the first results of a method to extract and sequence mature enamel proteins from modern human and Neanderthal tooth enamel. Using MALDI-TOF/TOF mass spectrometry and a combination of direct sequencing and peptide mass mapping we have sequenced a peptide from the tyrosine-rich amelogenin peptide (TRAP) of the X isoform of the amelogenin protein for modern and recent human samples...

MALDI-TOF-MS analysis of protein and DNA.

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has become a powerful and widespread analytical tool in all fields of life science. The wide mass range (1-300 kDa), high accuracy, and sensitivity make it a superior method for analysis of all kinds of biomolecules including proteins, nucleic acids, and carbohydrates. In combination with 2D-gelelectroph...

Characterization of fengycin homologues produced by Bacillus subtilis K1 by MALDI-TOF tandem mass spectrometry (MALDI TOF MS/MS)